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anti p stat3  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti p stat3
    Anti P Stat3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1296 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+stat3/Stat3+Rabbit+mAb/pm42817007-337-38-40
    Average 96 stars, based on 1296 article reviews
    anti p stat3 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: O-GlcNAcylation regulates microglial neuroinflammation in Parkinson's disease.
    Article Snippet: Western Blotting AR TI CL E IN P RE SS Equal amounts of protein (30 μg) were separated on 10% or 15% SDS-PAGE gels and transferred to nitrocellulose membranes (Amersham). .. Membranes were blocked with 5% nonfat milk in PBST for 1 h at room temperature, then incubated overnight at 4°C with primary antibodies (1:1,000) against various targets, including O-GlcNAc (sc-59623, RL2, Santa Cruz, Dallas, TX, USA), OGT (sc-74546, Santa Cruz), OGA (14771-1-AP, Proteintech, Rosemont, IL, USA), GFAT2 (A15374, Abclonal, Woburn, MA, USA) tyrosine hydroxylase (T2928,TH, Sigma-Aldrich), β-actin (sc28365, Santa Cruz), p-p65 (ab86229, Abcam, Cambridge, UK), p65 (sc-8008, Santa Cruz), p-IκB (sc-8404, Santa Cruz), IκB (sc-371, Santa Cruz), iNOS (610328, BD Bioscience, San Jose, CA, USA), COX2 (A3560, Abclonal), p-CREB (ab32096, Abcam), CREB (sc-377154, Santa Cruz), TLR4 (A0007, Abclonal), Arg1 (A4923, Abclonal), CD163 (A26411PM, Abclonal), CD86 (A16805, Abclonal), CD63 (A6554, Abclonal), c-Rel (sc-6955, Santa Cruz), p-STAT3 (9145, Cell Signaling Technology, Danvers, MA, USA), STAT3 (sc-8019, Santa Cruz), p-ERK (9101, Cell Signaling Technology), ERK (A16686, Abclonal), PPARγ (sc-7273, Santa Cruz), NLRP3 (sc134306, Santa Cruz), ASC (sc-514414, Cell Signaling Technology), cleaved caspase 1 (89332, Cell Signaling Technology), and caspase 1 (24232, Cell Signaling Technology). .. Membranes were washed and incubated with horseradish peroxidase-conjugated secondary antibodies (Invitrogen) for 1 h at RT.

    Blocking Assay:

    Article Title: Liver Kinase B1 in CD11c + Cells Inhibits Fibrosis in Chronic Pancreatitis via the Oncostatin M Signaling.
    Article Snippet: Proteins extracted from CD11c+ CD206+ cells and CD11c+ CD206− cells were subjected to electrophoresis on a 10% Tris SwePAGE gel (Servicebio, #G2302-10) and transferred onto a PVDF membrane. .. After blocking with 5% milk, the membranes were immunoblotted using the following antibodies: Lkb1 (1:1000; Cell Signaling, #3047), STAT3 (1:1000; Cell Signaling, #12 640), p-STAT3 (1:1000; Cell Signaling, #9134), JAK2 (1:1000; Cell Signaling, #3230), p-JAK2 (1:1000; Cell Signaling, #3776), CCL2 (1:1000; Abcam, #ab25124), α-SMA(1:1000; Abcam, ab5694) and GAPDH (1:1000; Aksomics, #KC-5G4). ..

    other:


    Article Title: Shengxian decoction mitigate bleomycin-induced pulmonary fibrosis in mice via MerTK mediated macrophage efferocytosis.
    Article Snippet: Ethnopharmacological relevance: Shengxian Decoction (SXD) is a classical multi-herb prescription widely used in traditional Chinese medicine for chronic respiratory ailments.. However, its pharmacological rationale and proresolving actions in idiopathic pulmonary fibrosis (IPF) have not been fully clarified.. Aim of the Study: This study investigated the anti-fibrotic efficacy of SXD in a bleomycin (BLM)-induced mouse model and explored whether MerTK-dependent macrophage efferocytosis contributes to SXD-driven resolution.

    Electrophoresis:

    Article Title: JAK1/2 inhibitor ruxolitinib reduces aggregates in cardiac proteinopathy.
    Article Snippet: Afterward, 8–25 μg protein with 6x Laemmli buffer was loaded onto 4–15% or 4–20% MiniPROTEAN TGXTM Precast Protein Gels (Bio-Rad, 4561084) or 8 or 12% self-casted acrylamide/bisacrylamide (29:1) gels. .. After 20 EMBO Molecular Medicine © The Author(s) electrophoresis, the proteins were transferred to nitrocellulose or PVDF membranes with Bio-Rad Tris/Glycine buffer or self-made transfer buffer (25 mM Tris base, 190 mM glycine, 20% methanol, pH 8.3) and stained with the following antibodies: JAK1 (R&D Systems, MAB4260); STAT3 (Cell Signaling Technology, 12640); P-STAT3 (Cell Signaling Technology, 9145S); GAPDH (HyTest, 5G4 or Sigma-Aldrich, P0067); ACTN2 (Sigma-Aldrich, A7811); GFP (Santa Cruz Biotechnology, sc-9996); LC3B (Cell Signaling Technology, 2775); p70 S6K (Cell Signaling, 34475); CRYAB (Enzo Life Sciences, ADI-SPA-222-F or Santa Cruz, sc-137129); desmin (Sigma-Aldrich, SAB5600054); FLAG (Sigma-Aldrich, F7425); ubiquitin (Enzo Life Sciences, BML-PW 8810). .. Western blots were detected with LI-COR Biosciences Odyssey, Biorad ChemiDoc, or Vilber Fusion FX7.

    Staining:

    Article Title: JAK1/2 inhibitor ruxolitinib reduces aggregates in cardiac proteinopathy.
    Article Snippet: Afterward, 8–25 μg protein with 6x Laemmli buffer was loaded onto 4–15% or 4–20% MiniPROTEAN TGXTM Precast Protein Gels (Bio-Rad, 4561084) or 8 or 12% self-casted acrylamide/bisacrylamide (29:1) gels. .. After 20 EMBO Molecular Medicine © The Author(s) electrophoresis, the proteins were transferred to nitrocellulose or PVDF membranes with Bio-Rad Tris/Glycine buffer or self-made transfer buffer (25 mM Tris base, 190 mM glycine, 20% methanol, pH 8.3) and stained with the following antibodies: JAK1 (R&D Systems, MAB4260); STAT3 (Cell Signaling Technology, 12640); P-STAT3 (Cell Signaling Technology, 9145S); GAPDH (HyTest, 5G4 or Sigma-Aldrich, P0067); ACTN2 (Sigma-Aldrich, A7811); GFP (Santa Cruz Biotechnology, sc-9996); LC3B (Cell Signaling Technology, 2775); p70 S6K (Cell Signaling, 34475); CRYAB (Enzo Life Sciences, ADI-SPA-222-F or Santa Cruz, sc-137129); desmin (Sigma-Aldrich, SAB5600054); FLAG (Sigma-Aldrich, F7425); ubiquitin (Enzo Life Sciences, BML-PW 8810). .. Western blots were detected with LI-COR Biosciences Odyssey, Biorad ChemiDoc, or Vilber Fusion FX7.

    Ubiquitin Proteomics:

    Article Title: JAK1/2 inhibitor ruxolitinib reduces aggregates in cardiac proteinopathy.
    Article Snippet: Afterward, 8–25 μg protein with 6x Laemmli buffer was loaded onto 4–15% or 4–20% MiniPROTEAN TGXTM Precast Protein Gels (Bio-Rad, 4561084) or 8 or 12% self-casted acrylamide/bisacrylamide (29:1) gels. .. After 20 EMBO Molecular Medicine © The Author(s) electrophoresis, the proteins were transferred to nitrocellulose or PVDF membranes with Bio-Rad Tris/Glycine buffer or self-made transfer buffer (25 mM Tris base, 190 mM glycine, 20% methanol, pH 8.3) and stained with the following antibodies: JAK1 (R&D Systems, MAB4260); STAT3 (Cell Signaling Technology, 12640); P-STAT3 (Cell Signaling Technology, 9145S); GAPDH (HyTest, 5G4 or Sigma-Aldrich, P0067); ACTN2 (Sigma-Aldrich, A7811); GFP (Santa Cruz Biotechnology, sc-9996); LC3B (Cell Signaling Technology, 2775); p70 S6K (Cell Signaling, 34475); CRYAB (Enzo Life Sciences, ADI-SPA-222-F or Santa Cruz, sc-137129); desmin (Sigma-Aldrich, SAB5600054); FLAG (Sigma-Aldrich, F7425); ubiquitin (Enzo Life Sciences, BML-PW 8810). .. Western blots were detected with LI-COR Biosciences Odyssey, Biorad ChemiDoc, or Vilber Fusion FX7.



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    ABclonal Biotechnology p stat3 tyr705
    A Representative images of placental villous explants cultured in rEDIL3 WT and rEDIL3 T88A groups. The bar represents 50 μm. B Western blot analysis of <t>STAT3</t> <t>and</t> <t>p-STAT3</t> expression, as well as the expression of EMT markers (E-cadherin, N-cadherin, Vimentin and Snail). C Transwell assay analysis of cell invasion and migration ability of HTR8/SVneo cells. D Bright field images of tube formation. E Western blot analysis of angiogenesis-related protein (CD31, CD34, VE-cadherin, VEGFR2 and VEGFA) expression. F CAM assay analysis of vascular formation. Data ( A , C , D , F ) are presented as mean ± SEM (3 independent biological replicates). Two-tailed unpaired Student’s t test. * p < 0.05, ** p < 0.01, *** p < 0.001. Source data are provided as a Source Data file.
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    Image Search Results


    Journal: Cell Reports Medicine

    Article Title: A biomimetic hybrid membrane vesicle nanoplatform attenuates tendinopathy through neuroinflammation modulation and tendon regeneration

    doi: 10.1016/j.xcrm.2026.102937

    Figure Lengend Snippet:

    Article Snippet: Rabbit Polyclonal anti-p-STAT3 , ABclonal , Cat# AP0705; RRID: AB_2863810.

    Techniques: Recombinant, Lysis, Hydroxyproline Assay, Enzyme-linked Immunosorbent Assay, Isolation, SYBR Green Assay, Bicinchoninic Acid Protein Assay, Staining, Software

    A Representative images of placental villous explants cultured in rEDIL3 WT and rEDIL3 T88A groups. The bar represents 50 μm. B Western blot analysis of STAT3 and p-STAT3 expression, as well as the expression of EMT markers (E-cadherin, N-cadherin, Vimentin and Snail). C Transwell assay analysis of cell invasion and migration ability of HTR8/SVneo cells. D Bright field images of tube formation. E Western blot analysis of angiogenesis-related protein (CD31, CD34, VE-cadherin, VEGFR2 and VEGFA) expression. F CAM assay analysis of vascular formation. Data ( A , C , D , F ) are presented as mean ± SEM (3 independent biological replicates). Two-tailed unpaired Student’s t test. * p < 0.05, ** p < 0.01, *** p < 0.001. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Defective O-fucosylation of EDIL3 attenuates invasion and vascular remodeling of trophoblasts via LIFR signaling pathway in preeclampsia

    doi: 10.1038/s41467-026-73139-4

    Figure Lengend Snippet: A Representative images of placental villous explants cultured in rEDIL3 WT and rEDIL3 T88A groups. The bar represents 50 μm. B Western blot analysis of STAT3 and p-STAT3 expression, as well as the expression of EMT markers (E-cadherin, N-cadherin, Vimentin and Snail). C Transwell assay analysis of cell invasion and migration ability of HTR8/SVneo cells. D Bright field images of tube formation. E Western blot analysis of angiogenesis-related protein (CD31, CD34, VE-cadherin, VEGFR2 and VEGFA) expression. F CAM assay analysis of vascular formation. Data ( A , C , D , F ) are presented as mean ± SEM (3 independent biological replicates). Two-tailed unpaired Student’s t test. * p < 0.05, ** p < 0.01, *** p < 0.001. Source data are provided as a Source Data file.

    Article Snippet: The membranes were incubated with blocking buffer containing 5% BSA (CAS:9048-46-8, Solarbio, China) and probed with the following primary antibodies at 4 °C overnight: poFUT1 (Cat No.14929-1-AP, Proteintech, 1:1000), EDIL3 (Cat No.12580-1-AP, Proteintech, 1:1000), His (Cat No.66005-1-Ig, Proteintech, 1:1000), CD31 (Cat No.11265-1-AP, Proteintech, 1:1000), CD34 (Cat No.BS45128, Bioworld, 1:1000), E-cadherin (Cat No.20874-1-AP, Proteintech, 1:1000), N-cadherin (Cat No.22018-1-AP, Proteintech, 1:1000), Vimentin (Cat No.10366-1-AP, Proteintech, 1:2000), Snail (Cat No.13099-1-AP, Proteintech, 1:1000), VE-cadherin (Cat No.sc-515467, SantaCruz, 1:500), VEGFR2(Cat No. 26415-1-AP, Proteintech, 1:1000), VEGFA (Cat No.sc-7269, SantaCruz, 1:500), PCNA (Cat No.AF0261, Beyotime, 1:1000), Cyclin D1 (Cat No.AF0126, Beyotime, 1:1000), Cyclin E1 (Cat No.AF6384, Beyotime, 1:1000), SQSTM1 (Cat No.18420-1-AP, Proteintech, 1:1000), LAMP2A (Cat No.66301-1-Ig, Proteintech, 1:1000), HSPA8 (Cat No.66442-1-Ig, Proteintech, 1:1000), ACTB (Cat No.20536-1-AP, Proteintech, 1:3000), p-STAT3 Tyr705 (Cat No.AP0705, Abclonal, 1:1000), STAT3 (Cat No.A19566, Abclonal, 1:1000), LIFR (Cat No.22779-1-AP, Proteintech, 1:1000) and GAPDH (Cat No.10494-1-AP, Proteintech, 1:1000).

    Techniques: Cell Culture, Western Blot, Expressing, Transwell Assay, Migration, Chick Chorioallantoic Membrane Assay, Two Tailed Test